The overarching aim of this project is to develop an innovative templating crystallisation platform for peptides. Specific objectives:
1-Investigate feasibility of peptides crystallisation using functionalised nano-templates. Hanging drop assays are performed to screen for crystallisation conditions (e.g., pH, buffer, salt types and concentrations, and nano-templates) using crystallisation robots (e.g., Mosquito).
2-Optimise the crystallisation conditions to improve peptides crystallisation (e.g. shorter induction times, crystallisation from lower concentrations, higher yields, particle properties, etc.) using PAT tools such as in-situ FTIR, UV-Vis and EasyViewer.
3-Multifactorial analysis for the identification of key process parameters (solvent properties, degree of supersaturation, cooling rates, stirring conditions, vessel size) and quantification of their impact on crystallisation and the resulting crystal properties (CSD, crystal shape, polymorphic forms). Development of digital twin to serve for in-silico optimisation. 4-Crystallisation is carried out at larger scales, moving from microliters to 10-100’s mL scales. Crystallisation development is undertaken using our Automated High-throughput Platform Suite (ATLAS).
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