Author: jgavira
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DC5 – Strategies for a fine-tuned control of biomolecules crystallisation
This project aims to achieve a comprehensive understanding of how electrical protocols can be designed to control the chemical and thermal environments during the crystallisation process. Specific objectives: 1-Understand the mechanisms by which ohmic heating influence protein solubility, nucleation and crystal development using well characterised protein systems of biological or structural importance. 2-Evaluate the impact…
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DC4 – Biomolecules aggregation and crystallisation in controlled flow/mixing regimes
The project aims at assessing the influence of different flow/mixing regimes on biomolecules crystallisation/nucleation process. Specific objectives: 1-Implement different controlled flow and mixing regimes in microfluidic setups. Verification of the dedicated flows with a novel 3D particle image velocimetry technique. 2-Screen for crystallisation conditions of the selected biomolecules applying counter diffusion methods. 3-Develop a flow…
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DC2 – Effect of external fields on the induction of nucleation
This project aims at studying the effect of an external field on biomolecules nucleation by applying an electric field or a laser pulse (NPLIN) into a microfluidic set-up. Globular proteins will be studied. Specific objectives: 1-Develop a microfluidic platform for triggering biomolecule crystallisation using an external field: electric field and/or laser pulse. 2-Measure statistical data…
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We are HIRING!!!
We have set up this network to help train the next generation of doctors to go beyond current practises and to respond to the near future biopharma manufacturing needs.
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DC3 – Screening of process conditions and external stimuli for biomolecules crystallisation in microfluidic devices
This project aims to develop efficient screening workflows for biomolecules relevant to therapeutic purposes (peptides). A droplet-based microfluidic screening platform will be developed. The experimental work is complemented by advanced modelling tools (e.g. COSMO-RS) to rationally select nucleating agents for a desired compound. Specific objectives: 1-Identify efficient crystallisation screening approaches applying nucleation promoting agents and…
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DC1 – Crystallisation of peptides in small volumes (several μL to 1 mL) applying designed templates or seeds at different solutions and additives conditions
The present project aims to contribute to a better understanding of the crystallisation of peptides and enable the development of strategies for crystallisation control on more representatives. Specific objectives: 1-Design of nucleating agents based on short amphiphilic peptides that self-assembly in ordered nano-complexes. 2-Investigate the influence of several approaches (i.e., solvent, nucleating agents, decoupling of…