Category: Jobs
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DC5 – Strategies for a fine-tuned control of biomolecules crystallisation
This project aims to achieve a comprehensive understanding of how electrical protocols can be designed to control the chemical and thermal environments during the crystallisation process. Specific objectives: 1-Understand the mechanisms by which ohmic heating influence protein solubility, nucleation and crystal development using well characterised protein systems of biological or structural importance. 2-Evaluate the impact…
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DC4 – Biomolecules aggregation and crystallisation in controlled flow/mixing regimes
The project aims at assessing the influence of different flow/mixing regimes on biomolecules crystallisation/nucleation process. Specific objectives: 1-Implement different controlled flow and mixing regimes in microfluidic setups. Verification of the dedicated flows with a novel 3D particle image velocimetry technique. 2-Screen for crystallisation conditions of the selected biomolecules applying counter diffusion methods. 3-Develop a flow…
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DC2 – Effect of external fields on the induction of nucleation
This project aims at studying the effect of an external field on biomolecules nucleation by applying an electric field or a laser pulse (NPLIN) into a microfluidic set-up. Globular proteins will be studied. Specific objectives: 1-Develop a microfluidic platform for triggering biomolecule crystallisation using an external field: electric field and/or laser pulse. 2-Measure statistical data…
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DC3 – Screening of process conditions and external stimuli for biomolecules crystallisation in microfluidic devices
This project aims to develop efficient screening workflows for biomolecules relevant to therapeutic purposes (peptides). A droplet-based microfluidic screening platform will be developed. The experimental work is complemented by advanced modelling tools (e.g. COSMO-RS) to rationally select nucleating agents for a desired compound. Specific objectives: 1-Identify efficient crystallisation screening approaches applying nucleation promoting agents and…
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DC1 – Crystallisation of peptides in small volumes (several μL to 1 mL) applying designed templates or seeds at different solutions and additives conditions
The present project aims to contribute to a better understanding of the crystallisation of peptides and enable the development of strategies for crystallisation control on more representatives. Specific objectives: 1-Design of nucleating agents based on short amphiphilic peptides that self-assembly in ordered nano-complexes. 2-Investigate the influence of several approaches (i.e., solvent, nucleating agents, decoupling of…